Inhibitory effect of Astragalus Polysaccharide combined with cisplatin on cell cycle and migration of nasopharyngeal carcinoma cell lines

Background Astragalus polysaccharide (APS) had shown great promise in anti-tumour activities our previous studies. The present study was designed to investigate whether APS has synergistic effect with cisplatin on the growth-inhibitory of human nasopharyngeal carcinoma cell lines and possible mechanism. Methods Here, (CNE-1) were divided into CNE-1 group, Cisplatin treatment group (2 µg/mL Cisplatin), (200 APS) combination 200 APS). proliferation inhibition rate cells determined by Cell Counting Kit-8 (CCK-8) method after different concentrations for 24, 48, 72 h. Apoptosis rates cycle retardation detected flow cytometry. migration invasion evaluated transwell assay. Western blotting quantitative (q)RT-PCR performed detect expression Bcl-2, Bax, caspase-3, matrix metalloproteinase-2 (MMP-2), p53 metalloproteinase-9 (MMP-9) proteins cells. Results have an time dose dependence manner. Both therapy could promote apoptosis cells, count increased G0/G1 S phase while decreased G2/M phase, inhibited Moreover, co-administration more efficacious antitumor than either agent alone, as evidenced significant decrease MMP-9 level increase p53. Conclusion APS, cisplatin, significantly lines, which may be related induction.